To extend these cellular studies on GIT1 to an in vivo system, we generated mice with globally inactivated Git1 gene by breeding mice carrying a conditional Git1(flox) allele with mice expressing the CMV-Cre transgene. Although many GIT1 knockout (GIT1-KO) animals died shortly after birth, homozygous mutants that survived the early postpartum period developed normally into adulthood and were fertile. Behavioral analyses of adult GIT1-KO mice revealed normal exploratory, anxiety- and depressive-like AZD3965 behaviors. However, GIT1-KO mice show impaired responses to fear conditioning and fear-potentiated startle.
Overall, these findings suggest that GIT1 is involved in the regulation of amygdala-mediated experience-based emotional behaviors. (c) 2009 Elsevier Ireland Ltd. All rights reserved.”
“Current knowledge of the central nervous system distribution of the beta(1)-adrenergic receptors (beta(1)-AR) is incomplete. Here we present a general map of the beta(1)-AR distribution in the rat brain. beta(1)-AR-immunoreactivity was detected throughout the entire rat brain, but particularly dense staining was observed in the cerebellar cortex and basal ganglia. Brainstem areas displaying significant beta(1)-AR-immunoreactivity
include through the ventrolateral medulla, nucleus ambiguus and the nucleus of the solitary tract. Within the hypothalamus, only the paraventricular nucleus Talazoparib solubility dmso and the median eminence (ME) showed beta(1)-AR immunostaining. Numerous beta(1)-AR-immunoreactive cells were also found in the hippocampus, basal ganglia and cerebral cortex. These results extend our knowledge of the expression profile of beta(1)-AR in the central nervous system. The identification of several distinct beta(1)-AR immunoreactive substrates linked with
neuropathophysiological roles in cardiovascular disease supports the hypothesis that the therapeutic benefit of beta(1)-AR blockade may be conferred at least in part through central nervous system mechanisms. (c) 2009 Elsevier Ireland Ltd. All rights reserved.”
“Background Statins lower high-sensitivity C-reactive protein (hsCRP) and cholesterol concentrations, and hypothesis generating analyses suggest that clinical outcomes improve in patients given statins who achieve hsCRP concentrations less than 2 mg/L in addition to LDL cholesterol less than 1.8 mmol/L (<70 mg/dL). However, the benefit of lowering both LDL cholesterol and hsCRP after the start of statin therapy is controversial. We prospectively tested this hypothesis.