The proceedings are published online in Advances in Dental Research
(2014, Vol. 26).”
“The relaxation mechanisms of tetrandrine (Tet) on the rabbit corpus cavernosum tissue in vitro were investigated. Strips of rabbit corpus cavernosum were mounted in organ chambers. The effects of Tet were examined on isolated muscle strips pre-contracted with phenylephrine (PE) alone, in the presence of NW-nitro-L-arginine (LNNA, a nitric oxide synthase inhibitor), 1-H-[1,2,4]oxadiazolo[4,3-]quinoxalin-1-one(ODQ, a guanylyl cyclase inhibitor), indomethacin (cyclooxygenase inhibitor), tetraethylammonium (TEA, Ca2+-activated K+ channel blocker), 4-aminopiridine (4-AP, voltage p38 MAPK inhibitor review dependent K+
channel blocker) Crenigacestat and glibenclamide (ATP sensitive K+channel blocker). The effects of Tet on KCl-induced contraction of isolated muscle strips were also investigated. The procedure of calcium absence-calcium addition was designed to observe the effect of Tet on the two components of the contractile responses to PE based on the source of Ca2+ (extracellular vs. intracellular). Corpus cavernosum strips showed relaxation in response to Tet (10-8 10-3 mol L-1) in a concentration-dependent manner with an IC50 of 3.73 10-5 mol L-1. However, they were not affected by LNNA, ODQ, indomethacin and K+-channel blockers. Tet (10 mol L-1, 30 mol L-1) concentration dependently reduced the maximal contraction response of isolated strips induced by KCl to (73.0 3.8) and (41.5 find more 3.4)%, respectively (p 0.01). In the procedure of calcium absence-calcium addition, Tet 100 mol L-1 inhibited both intracellular
calcium-dependent and extracellular calcium-dependent contraction induced by PE (20 mol L-1) (p 0.05). The inhibition ratios were (23.8 7.1) and (40.7 11.2)%, respectively. The results of the present study suggest that Tet possesses a relaxant effect on rabbit corpus cavernosum tissues, which is attributable to the inhibition of extracellular Ca2+ influx and the inhibition of release of intracellular-stored Ca2+, but not mediated by the release of nitric oxide, prostaglandins or by the activation of potassium channels.”
“Two novel secoiridoid glucosides, tripterospermumcins C (1) and D (2), were isolated from the aerial parts of Tripterospermum chinense, along with four known compounds, tripterospermumcin B (3), sweroside (4), loganic acid (5), and 8-epi-kingiside (6). Their structures were determined by analysis of 1D and 2D NMR data, as well as by comparison with model compounds. Compound 1 was a rare iridoid tetramer with four glucosides.”
“Contents Different culture conditions have been used to produce domestic cat embryos. As part of the in vitro procedures, the medium composition significantly affects the quality of the embryo development also.