However, none of the assessed neurophysiologic parameters was fou

However, none of the assessed neurophysiologic parameters was found to assist in the diagnosis of the sacral upper motor neuron lesion in individual patients. The shortened sacral reflex latency found in individual patients is there for enotacon sequence of a suprasegmentallesion, but rather of the low position of the conus medullaris (e.g., in tethered cord syndrome). Neurourol. Urodynam. 30:587-592, 2011. (C) 2011 Wiley-Liss, Inc.”
“Purpose: To develop a stability indicating RP-HPLC method for a combination drug product containing a high dose of paracetamol (PR) and low doses of domperidone

(DM) and tramadol HCL (TR).

Methods: The analytes are well separated by a reverse phase column and an isocratic mobile phase consisting of 0.1 % v/v trifluoroacetic find more acid: acetonitrile: methanol in the ratio 70: 25: 5 (v/v) with a flow rate of 1.0 mL/min. The effluent was monitored at 272 nm. The drug products were subjected to stress conditions of acid, base, peroxide, thermal and photolytic degradation and peak homogeneity of PR, TR and DM were obtained using photo diode array detector.

Results: The degradation products were well resolved from PR, TR and DM peaks, thus indicating the stability-indicating nature of the method. The assay was linear from

165 – 495 mu g/mL for PR, 18.75 – check details 56.25 mu g/mL for TR, and 5 – 15 mu g/mL for DM. Although the tablet contained high and low doses of the drugs, the intra-and inter-day variations were < 2.0 %.

Conclusion: The proposed method was validated according to the ICH guidelines and proved suitable for stability and homogeneity testing, as well as for quality control of the combined drugs in pharmaceutical preparations.”
“HER2 proto-oncogene encodes a transmembrane receptor tyrosine kinase overexpressed in a variety of solid tumors. Several mouse monoclonal antibodies (MAbs) have been developed that recognize the extracellular part of HER2; of them two MAbs

were humanized and employed for targeted immunotherapy. In this study we aimed to produce murine MAbs that specifically recognize the extracellular domain of human HER2. BALB/c mice were first primed with HER2-transfected NIH-3T3 cells and then boosted with recombinant extracellular part of HER2. Splenocytes from hyperimmunized mice were fused with myeloma cells and growing hybridomas were selected and screened for HER2 reactivity by an indirect ELISA. HER2-specific hybridomas were selected, cloned by limiting dilution assay, and further characterized by Western blotting and flow cytometry techniques. All clones showed positive reactivity to HER2 with binding affinity, ranging from 1.9 x 10(8) to 5 x 10(9), and stained HER2-transfected cells and malignant cells overexpressing HER2. None of the MAbs inhibited the binding of trastuzumab (Herceptin (R)) to HER2, indicating recognition of distinct epitopes by these MAbs.

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