The percentage of inhibition of ferrozine-Fe2+ complex formation was given in the underneath formula. Ferrousionschelatingability(%)=[(A0−A)/A0]×100Where,

A0 is the absorbance of the control solution (containing all reagents except plant extract); A is the absorbance in the presence of the sample of plant extracts. Three replicates were made for each test sample and average data was noted. Here, EDTA was used as positive control standard. The total phenolic contents of the extracts were determined by the modified Folin–Ciocaltu method.14 Briefly, 0.5 ml of each extract (1 mg/ml) was mixed with 5 ml Folin–Ciocaltu reagent (1:10 v/v distilled GSK J4 in vitro water) and 4 ml (75 g/L) of Sodium carbonate. The mixture was vortexed for 15 s and allowed to stand for 30 min at 40 °C for color development. The absorbance was read at 765 nm with a spectrophotometer see more (UV-1800, Shimadzu, Japan). Total phenolic content was determined as mg of gallic acid equivalent per gram using the equation obtained from a standard gallic acid calibration curve. For antioxidant determination, data were presented as mean ± Standard deviation (SD). Statistical analysis for animal experiment was carried out using one-way ANOVA followed by Dunnett’s multiple comparisons using SPSS 16.0 for Windows®. The results obtained were

compared with the control group. p values < 0.05 were considered to be statistically significant. A dose-dependent analgesic potential was showed by the crude extract of A. conyzoides and M. cordifolia leaves ( Table 1). The analgesic activities of both plants were significant (p < 0.05) at the dose of 500 mg/kg-body weight in comparison with control

animals; however, the activity was less than that of diclofenac Na (standard). In the study, A. conyzoides extract was found more effective Metalloexopeptidase than that of M. cordifolia L. The investigation shows that DPPH free radical scavenging activity of crude ethanolic extracts of A. conyzoides and M. cordifolia leaves were found to be increased with the increase of concentrations of the extracts ( Fig. 1). The extracts exhibited 91.72 ± 0.053% and 85.12 ± 0.087% inhibition respectively at the concentration of 100 μg/ml, whereas standard Ascorbic acid (AA) and BHA showed 95.86 ± 0.031% and 93.099 ± 0.019% inhibition respectively at the same concentration. In the study, if the IC50 value is less than 30 μg/ml, be considered as strong scavenging activity; 30 ≤ IC50 ≤ 100 μg/ml as moderate, and IC50 > 100 μg/ml be considered as weaker activity. 15 Therefore, it can be revealed that A. conyzoides got strong free radical scavenging activity (IC50 (μg/ml) = 18.91 ± 0.085), whereas M. cordifolia got moderate scavenging activity (IC50 (μg/ml) = 39.81 ± 0.081).