The recombinase activating genes (RAG) are essential for Selleck IBET762 editing and revision of the antigen receptors. The overall purpose of these processes lies in diversifying the antigen receptor repertoire and in revising autoreactive receptors to prevent autoimmunity. Consequently, these enzymes become promoters of self-tolerance during lymphocyte differentiation.

Once T and B cells mature, RAG expression is turned off and the cells are released to the periphery. However, re-expression of RAG proteins and receptor revision have been reported in mature peripheral blood B cells from patients with autoimmune diseases such as systemic lupus erythematosus (SLE) and juvenile idiopathic arthritis.[1-4] In these studies re-expression of RAG correlated with CD5 expression and was found to be dependent on interleukin-6 (IL-6).[5-7] Albeit RAG re-expression in the autoimmune context may result

from abnormal B-cell activation, the molecular mechanisms enabling re-expression and consecutive rearrangement processes remain to be investigated. Important evidence for a role of Toll-like receptors (TLR) in B-cell-mediated disease comes from studies dealing with autoimmune disorders. In this context, a central role of TLR was demonstrated in promoting the expansion of autoreactive B cells and autoantibody production.[8-10] Moreover, patients with SLE display an elevated frequency of TLR9-expressing B cells[11, 12] and TLR9-reactive CD27– effector memory B cells.[13] Ureohydrolase Everolimus datasheet Nonetheless, it was also reported that TLR9 exerts tolerogenic effects in murine SLE[14] and that patients with defective TLR signalling display elevated frequencies of autoreactive B-cell receptors (BCR),[15] indicating that TLR might influence receptor editing. However, only recently a clinical trial using TLR9 agonists, e.g. phosphorothioate-modified CpG oligodeoxynucleotides (CpGPTO) as adjuvant was halted because severe autoimmune disease developed in one subject.[16]

This unexplained incident encouraged us to investigate the role of TLR9 in B-cell tolerance, i.e. its role in receptor revision. The use of human materials was approved by the local ethics committee and written consent was obtained from donors. Total B cells were isolated from peripheral blood mononuclear cells with anti-CD19 microbeads (Miltenyi Biotec, Bergisch-Gladbach, Germany) (purity 98·5 ± 1%). For Igκ+ B-cell purification Igλ+ B cells were depleted with anti-Igλ-phycoerythrin (PE) and anti-PE microbeads before selection of Igκ+ B cells with anti-CD19 microbeads (purity 99 ± 0·5%). IgM+, IgM−, CD27+ and CD27− B-cell fractions were isolated as described previously.[17] Plasmacytoid dendritic cells were isolated with anti-BDCA4 beads (Miltenyi Biotec). Culture medium contained 5–10% heat-inactivated autologous serum [or 10% fetal calf serum (FCS; Biochrom, Cambridge, UK) for immunoglobulin assays]. Thymus was homogenized in Trizol (Invitrogen, Karlsuhe, Germany) or RIPA buffer.