Neighbor Joining tree graphically viewed using the FigTree program http://tree.bio.ed.ac.uk/software/figtree/. Branched tips labeled with protein accession number followed by species name. Scale bar indicates 0.06 amino acid substitutions per site. Branches colors are fungi-brown, algae-green, Archaea-red, Proteobacteria
(alpha-pink, beta-magenta, delta-blue, gamma-purple), Cyanobacteria-torquoise, Firmicutes-yellow, Actinobacteria-red and all other Bacteria-black. (PDF 6 MB) Additional file 2: Supplemental Table S1. Sequence accession numbers, taxa name and sequence length of putative ChrA sequences used in phylogenetic analysis. (DOC 588 KB) Additional file 3: Supplemental Figure S2. Operon structure analysis of the Arthrobacter sp. strain FB24 CRD. RT-PCR was used to determine co-transcription of the genes within the chromate resistance selleck compound determinant. A: Location of primer pairs. Primer sequences are listed in table 4. Primer numbers correspond to the 17DMAG datasheet following primers: 1-MQO RT/A, 2-BC RT/A, 3-SP RT/F, 4-SP RT/R,
5-COG4RT/F, 6-COG4RT/R, 7-ChrAP RT/A, 8-ChrAP RT/B, 9-BP RT/R. B: RT-PCR results with listed primer pairs. C: RT-PCR products of reactions performed with primer pair 2 + 4 (lanes 2 and 3) and primer pair 5 + 8 (lanes 8 and 9). Lanes 1 and 7-100 bp PCR ruler, dark band is 1 kb; Lanes 4 and 10-no template controls; Wilson disease protein Lanes 5 and 11-No RT controls; Lanes 6 and 12 positive PCR control using pKH12 as template. (JPEG 32 KB) Additional file 4: Supplemental Table S2. Recipe for vitamin solution added to mXBM. (DOC 30 KB) References 1. Jones D, Keddie RM: The Genus Arthrobacter. The Prokaryotes: An Evolving Electronic Resource for the Microbiological
Community, release 3.0 edn 3 Edition (Edited by: Dworkin, et al). New York: Springer-Verlag 1999. 2. Crocker FH, Fredrickson JK, White DC, Ringelberg DB, Balkwill DL: Phylogenetic and physiological diversity of Arthrobacter strains isolated from unconsolidated subsurface sediments. selleck chemicals Microbiology 2000,146(Pt 6):1295–1310.PubMed 3. van Waasbergen LG, Balkwill DL, Crocker FH, Bjornstad BN, Miller RV: Genetic diversity among Arthrobacter species collected across a heterogeneous series of terrestrial deep-subsurface sediments as determined on the basis of 16S rRNA and recA gene sequences. Appl Environ Microbiol 2000,66(8):3454–3463.CrossRefPubMed 4. Benyehuda G, Coombs J, Ward PL, Balkwill D, Barkay T: Metal resistance among aerobic chemoheterotrophic bacteria from the deep terrestrial subsurface. Canadian Journal of Microbiology 2003,49(2):151–156.CrossRefPubMed 5. Margesin R, Schinner F: Heavy metal resistant Arthrobacter sp.–a tool for studying conjugational plasmid transfer between gram-negative and gram-positive bacteria. J Basic Microbiol 1997,37(3):217–227.CrossRefPubMed 6.