Infect Immun 2003,71(10):5724–5732.PubMedCrossRef check details 32. Donis-Keller H, Maxam AM, Gilbert W: Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res 1977,4(8):2527–2538.PubMedCrossRef 33. Pezzulo AA, Starner TD, Scheetz
TE, Traver GL, Tilley AE, Harvey B-G, Crystal RG, McCray PG Jr, Zabner J: The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia. Am J Physiol Lung Cell Mol Physiol 2011, 300:L25-L31.PubMedCrossRef 34. Lee HY, Takeshita T, Shimada J, this website Akopyan A, Woo JI, Pan H, Moon SK, Andalibi A, Park RK, Kang SH, et al.: Induction of beta defensin 2 by NTHi requires TLR2 mediated MyD88 and IRAK-TRAF6–MAPK signaling pathway in human middle ear epithelial cells. BMC Infect Dis 2008, 8:87.PubMedCrossRef 35. JQ1 mw Kwon AR, Kim JH, Park SJ, Lee KY, Min YH, Im H, Lee I, Lee KY, Lee BJ: Structural and biochemical characterization of HP0315 from Helicobacter pylori as a VapD protein with an endoribonuclease activity. Nucleic Acids Res 2012,40(9):4216–4228.PubMedCrossRef 36. Christensen SK, Mikkelsen M, Pedersen K, Gerdes K: RelE, a global inhibitor of translation, is activated during nutritional stress. Proc Natl Acad Sci U S A 2001,98(25):14328–14333.PubMedCrossRef 37. Jorgensen
MG, Pandey DP, Jaskolska M, Gerdes K: HicA of Escherichia coli defines a novel family of translation-independent mRNA interferases in bacteria and archaea. J Bacteriol 2009,191(4):1191–1199.PubMedCrossRef 38. Pedersen K, Christensen SK, Gerdes K: Rapid induction and reversal of a bacteriostatic condition by controlled expression
of toxins and antitoxins. Mol Microbiol 2002,45(2):501–510.PubMedCrossRef 39. Ahidjo BA, Kuhnert D, McKenzie JL, Machowski EE, Gordhan BG, Arcus V, Abrahams GL, Mizrahi V: VapC toxins from Mycobacterium tuberculosis are ribonucleases that differentially inhibit growth and are neutralized by cognate VapB antitoxins. PLoS One 2011,6(6):e21738.PubMedCrossRef 40. Ren D, Nelson KL, Uchakin PN, Smith AL, Gu XX, Daines DA: Characterization of extended co-culture of non-typeable Haemophilus ROS1 influenzae with primary human respiratory tissues. Exp Biol Med (Maywood) 2012,237(5):540–547.CrossRef 41. Lioy VS, Rey O, Balsa D, Pellicer T, Alonso JC: A toxin-antitoxin module as a target for antimicrobial development. Plasmid 2010,63(1):31–39.PubMedCrossRef 42. Herriott RM, Meyer EM, Vogt M: Defined nongrowth media for stage II development of competence in Haemophilus influenzae. J Bacteriol 1970,101(2):517–524.PubMed 43. Miller JH: Experiments in molecular genetics. Cold Spring Harbor: Cold Spring Harbor Laboratory Press; 1972. Competing interests The authors declare that they have no competing interests. Authors’ contributions DR drafted the manuscript, performed the mutagenesis, protein-protein interaction, and EpiAirway assays, and participated in the animal studies.