In order to overcome this problem, in the colonization study described here we serotyped up to ten isolates per child, selecting randomly and/or by isolate morphology in cases where morphological selleck chemicals llc differences were apparent. Until consensus on a more suitable method for the evaluation of the nasopharyngeal flora of pneumococci is reached, a recent study proposed serotyping

of multiple isolates selected on the basis of morphological variation plus random picking as a reasonable way of assessing the composition of the pneumococcal nasopharyngeal flora [15]. The World Health Organization and UNICEF have recognized the safety and effectiveness of PCV7, recommending the inclusion of this vaccine in national immunization programs.

Indeed, 35 high- and middle-income countries currently provide routine childhood immunization against pneumococcal disease, and Rwanda has recently become the first developing nation to introduce PCV7 [16]. However, in developing countries the current high price of the vaccine doses hinders the introduction of PCV7 [17]. There are reasons to believe that a single Crenolanib clinical trial PCV7 dose has the potential to prevent a significant amount of invasive pneumococcal disease in children [18] and [19]. As the nasopharynx is the launching pad for pneumococcal disease, it is also of utmost importance to understand the effect of one dose in this niche. If proven efficacious, the use of a single vaccine dose may reduce the cost of vaccination sufficiently to facilitate introduction of PCV7 in more developing countries. To our best knowledge, the efficacy of a single dose of PCV7 on single and multiple colonization has not been evaluated, and studies on the effect of fewer than the recommended doses are scarce [20], [21], [22] and [23]. This evaluation should rely not only on the pneumococcal prevalence comparison among vaccinated and control groups, but also on the identification of the actual mechanism of the next vaccine’s effect [24]. In this study we evaluated the impact of one PCV7 dose on single

and multiple pneumococcal colonization in a group of children attending day care centers, identifying the mechanisms of the vaccine’s effect. Eighty-five healthy children attending 5-day care centers in the Lisbon area of Portugal were enrolled in this observational study of the effect of a single dose of PCV7 on pneumococcal colonization. Vaccinated and control group allocation was based on three criteria—age between 12 and 24 months, same geographical area, and same social background. Children fulfilling the three requirements were included in the study. Those that were immunized with a single PCV7 dose (69 children) constituted the vaccinated group, and those that received no vaccine (16 children) formed the control group. In the vaccinated group, 38 children (55%) were males and 31 (45%) were females.