I will continue to study and report the relationship cytokines with TLR effect in the AAV. PARK HOON SUK, KIM EUN NIM, KIM MIN YOUNG, LIM JI HEE, YU JI HYUN, HWANG SEUN DEUK, PARK CHEOL WHEE, CHOI BUM SOON Division

of nephrology, Department of Internal medicine, The Catholic university of Korea Introduction: HMGB1 (High mobility group box1) is known to be an important mediator in inflammatory pathway. It is associated with ischemic insults in myocardial and cerebral infarction, so its blockade leads to protection from organ damages. We performed this study to see if the blocking of HMGB1 prevents chronic cyclosporine (CsA) toxicity in a mouse model. Methods: Male ICR mice (25 g) were used. Chronic CsA toxicity was caused by its subcutaneous (SC) injection daily for 4 weeks. Each group (n = 6) was respectively control group selleck compound (olive oil 1 mL/kg SC injection), CsA toxicity group (CsA 30 mg/kg SC injection), anti-HMGB1 group (anti HMGB1 chicken IgY antibody (600 mg/mouse) intraperitoneal (IP) injection weekly for blocking HMGB1) and non-specific IgY group (polyclonal non-specific chicken IgY antibody (600 mg/mouse) IP injection weekly). Results: Anti- HMGB1 group showed decreased 24 hour albuminuria (23.78 ± 8.06 mcg/day vs. 62.69 ± 28.83 mcg/day,

p = 0.03), increased creatinine clearance (0.12 ± 0.03 ml/min vs. 0.07 ± 0.02 ml/min, p = 0.05) and decreased serum creatinine level (0.22 ± 0.02 mg/dl vs. 0.33 ± 0.04 mg/dl, Dorsomorphin chemical structure p = 0.01), compared with CsA toxicity group. Tubular interstitial fibrosis area (2.19 ± 1.97% vs. 14.65 ± 6.54 %, p = 0.008) and TGF-beta immunohistochemical stain (11.47 ± 0.88 fold vs. 16.06 ± 4.81 fold, p = 0.05) were also decreased in anti-HMGB1 group vs. CsA toxicity group. 8 OHDG level in 24 hour urine was decreased, but was not significant (52.94 ± 15.34 mcg/day G protein-coupled receptor kinase in anti HMGB1 group vs. 72.45 ± 13.77 mcg/day in CsA group, p = 0.12). RAGE (0.74 ± 0.03 fold vs. 1.27 ± 0.29 fold, p = 0.02) and TLR4 (0.41 ± 0.09 fold vs. 0.89 ± 0.14 fold, p = 0.05),

which are known to interact with HMGB1, expressions were decreased in anti-HMGB1 group vs. CsA toxicity group. Conclusion: The administration of anti-HMGB1 brought renal functional improvements and ameliorated fibrosis induced by CsA and it is thought to result from decrease in TLR4 and RAGE expressions. JAIYEN CHALIYA1,2, JUTABHA PROMSUK1, ANZAI NAOHIKO1, SRIMAROENG CHUTIMA2 1Department of Pharmacology and Toxicology, Dokkyo Medical University, School of Medicine, Tochigi 321-0293, Japan; 2Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand Introduction: Green tea is famous beverage in Asia. It originally made from the leaves of Camellia sinensis. Green tea extract (GT) and its constituents exerted several biological activities, including anti-cancer, hepato-protective, and anti-oxidant actions.