(DOC 924 KB) Additional file 2 : Figure S2. Non-coverage rates at the phylum level. The figures show the non-coverage rates of different primers at the phylum level: A Primer 27F; B Primer 338F; C Primer 338R; D Primer 519F; E Protein Tyrosine Kinase inhibitor Primer 519R; F Primer 907R; G Primer 1390R; and H Primer 1492R. (DOC 214 KB) Additional file 3 : Table S1; Table S2; Table S3; Table S4; Table S5. Primer binding-site sequence variants. Frequently observed sequence

variants at different primer binding sites are listed in different tables: Table S1 Primer 27F; Table S2 Primer 338F; Table S3 Primer 338R; Table S4 Primer 519F; and Table S5 Primer 907R. (DOC 258 KB) Additional file 4 : Figure S3. Elimination of primer contamination. The figure shows the elimination of sequences that are thought to lack correct primer trimming in the Trichostatin A order RDP dataset. (DOC 463 KB) References 1. Olsen GJ, Lane DJ, Giovannoni SJ, Pace NR, Stahl DA: Microbial ecology and evolution: a ribosomal RNA approach. Annu Rev Microbiol 1986, 40:337–365.PubMedCrossRef 2. Schmidt TM, Delong EF, Pace NR: Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing. J Bacteriol 1991, 173:4371–4378.PubMed 3. Sharkey FH, Banat IM, Marchant R: Detection and quantification of

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