Dielectrophoresis (DEP) can discriminate and manipulate cancer
cells in microfluidic systems and has been proposed as a molecular marker-independent approach for isolating CTCs from blood. To investigate the potential applicability of DEP to different cancer types, the dielectric and density properties of the NCI-60 panel of tumor cell types have been measured by dielectrophoretic field-flow fractionation (DEP-FFF) and compared with like properties of the subpopulations of normal peripheral blood cells. We show that all of the NCI-60 cell types, regardless of tissue of origin, exhibit dielectric properties that SNS-032 facilitate their isolation from blood by DEP. Cell types derived from solid tumors that grew in adherent cultures exhibited dielectric properties that were strikingly different from those of peripheral blood cell subpopulations while leukemia-derived lines that grew in non-adherent cultures exhibited dielectric properties that were closer to those of peripheral blood cell types. Our results suggest CX-4945 in vitro that DEP methods have wide applicability for the surface-marker
independent isolation of viable CTCs from blood as well as for the concentration of leukemia cells from blood. (C) 2013 American Institute of Physics. [http://dx.doi.org.elibrary.einstein.yu.edu/10.1063/1.4774307]“
“In the present work, the effect of altitudinal variations on the total phenol, flavonoid, and antioxidant
potential of seeds and hull of Tartar buckwheat collected from 15 different locations of Western Himalaya has been investigated. Methanolic and acetonic extracts of seed and hull powder showed significantly increasing amounts of total polyphenol content, and antioxidant potential with rising altitude. Methanolic extract of seed and hull powder were found to have significantly greater polyphenol, and antioxidant potential as compared to acetonic extract. The methanolic seed extract of Inderdhara (3,133 m) showed the maximum total phenolic content 1,651 mu g gallic acid https://www.selleckchem.com/products/blasticidin-s-hcl.html equivalent/50 mg of dry weight. The methanolic seed extract of Inderdhara showed the maximum antioxidant activity (77.29+/-1.2%) and antiradical activity (55.5+/-0.5%) also. The results of this study exhibited good correlation with total polyphenol and antioxidant potential in all the samples followed by the increasing tendency towards rising altitude.”
“Circulating tumor cells (CTCs) are prognostic markers for the recurrence of cancer and may carry molecular information relevant to cancer diagnosis. Dielectrophoresis (DEP) has been proposed as a molecular marker-independent approach for isolating CTCs from blood and has been shown to be broadly applicable to different types of cancers. However, existing batch-mode microfluidic DEP methods have been unable to process 10 ml clinical blood specimens rapidly enough.