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“Background Although Mycobacterium smegmatis was originally isolated from humans, this fast-growing mycobacterium species is mostly Ipatasertib nonpathogenic and has been used as a model to investigate mycobacterial BB-94 physiology [1, 2]. This fast-growing nonpathogenic bacterium is

particularly useful in studying basic cellular processes of relevance to pathogenic mycobacteria, such as Mycobacterium tuberculosis, M. avium subsp. paratuberculosis and M. leprae, respectively the causative agent of tuberculosis, Johne’s disease and leprosy. Although the genome sequencing of M. smegmatis is completed, much is unknown about the mechanisms controlling growth in mycobacterial species. As occurs with all free living

bacteria, cells of M. smegmatis are surrounded by a cell wall responsible for providing their shape. The wall also provides protection to the cell to withstand the difference in osmotic pressure with the medium, and against other physical and chemical aggressions. Nevertheless, the cell wall must not be considered as a static structure; its chemical composition and the assembly of the different macromolecules that make it up are modified during cell growth and morphogenesis. A characteristic feature of mycobacteria is the thick, waxy cell wall, a highly impermeable outer surface, which enables mycobacteria to survive in extreme environmental Necrostatin-1 manufacturer conditions and the presence of antibiotics. The cell envelope structure of Mycobacteria is different from other gram positive bacteria, by the fact that it has two lipid layers, one being a regular inner membrane, the second being a layer mainly

consisting of mycolic acids. This mycomembrane is very tightly connected to the peptidoglycan and arabinomannan inner layers of the cell wall. The surface is very complex, composed of proteins, sugars, and lipids that are in part conserved across the Mycobacterial Thiamet G genus. While many of the cell wall proteins are burried inside the cell wall, some are surface exposed and likely play an even greater role in many vital processes such as cell-cell interactions, ion and nutrient transport and cell signaling, and participate in the key pathogenically relevant cellular mechanisms. Many proteins required for the pathogenicity of Mycobacteria are surface proteins that are involved in lipid metabolism and transport across the cell envelope [3, 4]. Surface proteins are exposed to the external environment. As a result, these proteins are ideally positioned to protect the bacterium or to modify the host immune response to the bacillus. So research on the cell wall proteome of M. smegmatis provides promising candidates for vaccine and drug development against pathogenic Mycobacterium spp., especially since it turns out that bacterial cell envelope together with plasma membrane proteins constitute the majority of currently known drug targets [5, 6].