99 The sensitivity of qPCR assays was 9 1 × 10-3, 1 5 × 10-4, 3

99. The sensitivity of qPCR assays was 9.1 × 10-3, 1.5 × 10-4, 3.7 × 10-4, 1.7 × 10-1, 1.4 × 10-2, 4.9 × 10-4, 3.3 × 10-1 ng of target DNA for Lactobacillus, Bifidobacterium, S. thermophilus,

G. vaginalis, Atopobium, find more Prevotella and Veillonella, respectively. All subjects naturally harbored strains belonging to Lactobacillus, Bifidobacterium, Atopobium and Prevotella, as demonstrated by the presence of these genera in the vaginal samples collected at W33. Woman N. 9 (P group) was the only exception lacking lactobacilli at both the baseline and EPZ004777 supplier after one-month intake of VSL#3 (Table 2). G. vaginalis was found in two women belonging to C group (N. 18 and 20) at both time points at the concentration of 5.5 × 101 ± 3.8 (N. 18: W33), 7.5 × 101 ± 4.6 (N. 18: W37), 2.2 × 102 ± 1.8 × 101 (N. 20: W33) and 1.9 × 102 ± 3.2 × 101 (N. 20: W37). S. thermophilus and Veillonella were not detected in GSK1838705A concentration any pregnant woman enrolled in this study. Statistical elaboration of qPCR data related to Lactobacillus, Bifidobacterium, Atopobium and Prevotella was performed to search for significant variations of these genera associated with the

going on of pregnancy or the probiotic supplementation (Figure 3). No significant changes in the amounts of these bacteria were found between W33 and W37 in both P and C groups. However, in spite of the lack of statistical relevance, a weak modulation was observed for Bifidobacterium and Atopobium. Regarding bifidobacteria (Figure 3B), a physiological tendency to decrease was observed in vaginal samples of control women at the end of the study period (mean value, W33: 4.3 MycoClean Mycoplasma Removal Kit ± 2.2 × 10-1; W37: 2.0 ± 1.7 × 10-1). This trend seemed to be counterbalanced in women consuming VSL#3 since amount of bifidobacteria slightly increased during the supplementation period (mean value, W33: 9.9 × 10-1 ± 1.6 × 10-1; W37: 1.4 ± 1.2 × 10-1). An opposite trend was observed for Atopobium (Figure 3C). This genus increased at W37 (mean value, 9.2 ± 3.2) compared to W33 (mean value, 7.0 ± 2.8) in C group, while it remained constant after VSL#3 supplementation (mean value, W33: 1.4 × 101 ± 3.8; W37: 1.3 × 101 ± 5.2). Table 2 qPCR data of Lactobacillus, Bifidobacterium, Atopobium

and Prevotella     ng of target DNA/μg vaginal genomic DNA (mean ± SD) Woman N. Time point Lactobacillus Bifidobacterium Atopobium Prevotella Probiotic (P)           1 W33 2.4 × 101 ± 1.1 1.9 × 10-2 ± 7.4 × 10-3 3.6 ± 1.5 2.1 × 10-2 ± 1.0 × 10-2   W37 3.0 × 101 ± 3.1 3.1 × 10-2 ± 2.7 × 10-4 1.3 × 101 ± 6.8 9.1 × 10-2 ± 1.6 × 10-2 2 W33 9.6 ± 8.7 × 10-1 3.1 × 10-2 ± 8.8 × 10-3 5.4 × 101 ± 7.4 1.4 × 10-1 ± 4.8 × 10-2   W37 5.9 × 10-1 ± 4.9 × 10-2 2.4 × 10-2 ± 1.2 × 10-2 2.4 × 101 ± 1.9 × 101 1.1 × 10-1 ± 1.1 × 10-2 3 W33 2.4 × 101 ± 2.9 2.4 × 10-2 ± 4.2 × 10-3 1.1 × 101 ± 6.0 1.1 × 10-1 ± 7.7 × 10-3   W37 2.2 × 101 ± 2.4 3.0 × 10-2 ± 2.4 × 10-3 4.0 ± 2.3 5.2 × 10-2 ± 8.2 × 10-3 4 W33 2.2 × 101 ± 2.0 6.8 × 10-2 ± 8.3 × 10-3 4.7 ± 1.9 7.3 × 10-2 ± 2.

Leave a Reply

Your email address will not be published. Required fields are marked *

*

You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <strike> <strong>