1 for GC recurrence prediction (sensitivity = 66.67 and specificity = 83.3). When combining methylated genes and the clinical
stage, the AUC was 83.69, with a sensitivity of 76.19 and a specificity of 83.3. Conclusion: The status of promoter hypermethylation of SFRP-1 and DKK-3 in plasma may serve as a non-invasive diagnostic and prognostic biomarker for GC. Key Word(s): 1. gastric cancer; 2. SFRP1; 3. DKK-3; 4. Plasma; Presenting Author: FENGTING HUANG Additional Authors: SHINENG ZHANG, ZHIQIANG GU Corresponding Author: SHINENG ZHANG Affiliations: Sun Yat-sen Memorial Hospital, Sun Yat-sen University Objective: Colorectal cancer is one of the most common cancers of selleck screening library human and with the increasing morbidity in China recent years. The long intergenic non-coding RNAs (lincRNAs) are emerging as promising regulatory factors in various cancers. In this study, we investigated lincRNAs profiles in 4 pairs of human colorectal cancers (CRC) and the corresponding adjacent nontumorous tissues (NT) by microarray. Methods: The expression of lincRNAs and mRNAs in both CRC and NT was detected by microarray assays. The lincRNAs and mRNAs that were differentially expressed (>2 fold-change or <0.5 fold-change) in CRC compared to NT were screened out.
The potential target genes of Selleckchem FDA-approved Drug Library the differentially expressed lincRNAs were predicted via cis-regulatory effects. Bioinformatic analysis was performed through gene ontology (GO) analysis. The pathway analysis showed the target gene-related pathways. Results: With abundant probes accounting 42545 RNAs in our microarray, 124 lincRNAs were differentially expressed selleck kinase inhibitor (>2 fold-change or <0.5 fold-change) in CRC tissues compared with NT, and 1633 mRNAs were differentially expressed. 41 potential
target genes of differentially expressed lincRNAs were predicted. GO analysis displayed various functions of the predicted target genes. 17 signal pathways were considered to be associated with these predicted target genes. Conclusion: Our study reveals genome-wide lincRNAs expression patterns in CRC by microarray. The results imply that clusters of lincRNAs are aberrantly expressed in CRC compared with NT, which suggests that lincRNAs may play a novel role in the pathogenesis and progression of CRC through different mechanisms and pathways. Taken together, our study may provide a promising insight of CRC. Key Word(s): 1. lincRNAs; 2. colorectal cancer; 3. pathogenesis; 4. progression; Presenting Author: LEI LI Additional Authors: TIAN LUO, WEI JIANG Corresponding Author: LEI LI Affiliations: zhongshan hospital, Fudan university Objective: Colorectal cancer (CRC) is a major cause of morbidity and mortality throughout the world. However, the genetic alterations and molecular mechanism of the early onset CRCs are not fully investigated.