, 2008; Yang et al., 2009; Zaparoli et al., 2009; Bernardi et al., 2011). The relation here found, and the fact that these widely different stresses and growth conditions all had much the same down-regulating effect on the transcription of cp, suggest that the regulation of cp was most likely not caused directly by the particular factor tested, but was a more general response www.selleckchem.com/products/PLX-4720.html to the growth level of the fungus. This hypothesis is supported by the similarity of the 3D structure of CP to expansins (de Oliveira et al., 2011), proteins mainly found in plants where they have various roles in growth and in developmental processes involving cell wall modifications (McQueen-Mason & Cosgrove, 1994;
Cosgrove et al., 2002; Li et al., 2003; Choi et al., 2006). A small number of expansin-like proteins has also been found in fungi (Saloheimo et al., 2002; Bouzarelou et al., 2008; Brotman et al., 2008; Chen et al., 2010; Wang et al., 2010; Quiroz-Castañeda et al., 2011). Expansins cause cell wall loosening and cellulose disruption even though they do not have any cellulose-hydrolytic activity. Like expansins, CP is localized in the cell
CHIR-99021 concentration wall, has a double-ψβ-barrel fold, lacks lytic activity and has the ability to bind oligosaccharides. Moreover, the residues involved in carbohydrate binding are conserved among the members of the CP family, suggesting that the biological function of these proteins could be related to polysaccharide binding (de Oliveira et al., 2011). In conclusion, our results strengthen the functional similarity between CP and expansins and allow us to propose the involvement of CP in the remodelling and enlargement of the Dichloromethane dehalogenase cell wall that occur during hyphal growth and in the formation and differentiation process of chlamydospores. The work was supported by the Ministero Italiano dell’Università e della Ricerca Scientifica, Progetti di
Ricerca di Interesse Nazionale 2007 to A.S. “
“Salmonella enterica serovar Enteritidis is a major cause of human gastrointestinal disease, infection being due in large part to consumption of contaminated eggs. The lipopolysaccharide (LPS) of Salmonella is known to play a role in colonisation of the host and survival in hostile conditions including egg albumen. We investigated the contribution of LPS O-antigen length to colonisation of the reproductive tract of laying hens, contamination of eggs and survival in albumen. We show that expression of very-long O-antigen is essential for contamination of eggs, probably as a consequence of enhanced reproductive tract colonisation and survival in the forming egg. “
“Phosphorothioate modification of DNA and the corresponding DNA degradation (Dnd) phenotype that occurs during gel electrophoresis are caused by dnd genes. Although widely distributed among Bacteria and Archaea, dnd genes have been found in only very few, taxonomically unrelated, bacterial species so far.