1 appears in the UniProt Knowledgebase under the accession number P86386. Inhibitory effect of mutacin F-59.1 One milliliter of active preparation (1600 AU/mL) adjusted
to pH 7.0 was filter sterilised then added to 10 mL of an early-log-phase culture of Micrococcus luteus ATCC 272 grown in TSBYE. Bacterial culture in TSBYE was used as a negative control. The viable count in CFU/mL was determined at intervals for up to 24 h for samples and control during incubation at 37°C by plating 100 μL of an appropriate dilution in peptone water (0.1%) on TSAYE incubated at 37°C at least 24 h. Acknowledgements This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC). We are grateful to Jean Barbeau of University of Montréal allowing selleck chemicals sequencing of mutacin D-123.1. We thank Alain Gaudreau of the STELA Dairy Research Center of Université selleck kinase inhibitor Laval for technical assistance in the purification process and France Dumas from the Biotechnology Research Institute
of Montréal for the sequencing procedure. We also thank Johnny Basso of University of Ottawa and Franck Stefani from Canadian Forest Service (Québec) for their critical review of the manuscript. Guillaume Nicolas is supported by a University-Industry Ph.D. Scholarship from NSERC and Microbio LCA Inc. Marc C. Lavoie is supported by a grant from the Caribbean Health Research Council to study mutacins. References 1. Fischbach MA, Walsh CT: Antibiotics for emerging pathogens. Science 2009, 325:1089–1093.PubMedCrossRef 2. Drider D, Fimland G, Héchard Y, McMullen LM, Prévost H: The mTOR inhibitor continuing story of class IIa bacteriocins. Microbiol Mol Biol Rev 2006, 70:5 64–82.CrossRef 3.
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