This suggests a realized heritability of 0.439, 0.571 and 0.518 from the single plant selection for seedling ST from the three BC2F2 populations. The initial screen for DT under the severe field drought conditions in Hainan resulted in 19 (4.0%), 29 (6.0%) and 33 (6.9%) plants with obviously higher fertility and GY than HHZ selected from the

HHZ/IR64, HHZ/AT354 and HHZ/C418 BC2F2 populations (Fig. 1). However, the severe drought in the progeny testing under the controlled conditions of the greenhouse in Beijing killed HHZ (no yield), but 12, 23 and 8 BC2F3 lines from the three populations survived and produced seeds, resulting in a realized heritability of 0.632, 0.793 and 0.242 from the single plant Selleck PFT�� selection for DT from the three BC2F2 populations in Hainan. When evaluated under the mild drought stress in Hainan during the 2011–2012 DS, 8 of the 43 DT selected ILs showed significantly higher GY than HHZ, and none of them had lower GY than HHZ (Table 1), indicating that the selection for DT was highly effective. When the 189 ILs were evaluated under drought stress and normal irrigated conditions of Hainan during the 2011–2012 DS, water treatments (T) had highly significant effect on SP600125 order all measured

traits, but this variation component varied considerably among different traits with R2 ranging from 2.3% for PN to 45.7% for FNP. On average, the yield reduction caused by the drought stress was 20% for HHZ (the recipient) but 36.1% for the 189 ILs. Differences among different ILs (G) were highly significant for all measured traits and accounted for an average 36.6% of the total trait variation, ranging from 26.7% for FNP to 53.9% for PH. The T × G interaction was insignificant

for all measured traits, indicating that all ILs performed consistently under drought stress and well watered conditions for the measured traits in this experiment. ANOVA also indicated that ILs from different populations showed significant differences for Tolmetin all measured traits except for PH, ranging from 2.3% for PN to 19.0% for GW. Similarly, different selection schemes had highly significant effects on the mean performances of the ILs for all traits except for SF and GY, ranging from 1.8% for PN to 38.4% for HD. Although all were highly significant, ILs selected from different populations (P) showed much greater trait variation than ILs obtained from different selection schemes (S). The P × S interaction was also significant for all measured traits, indicating that selection efficiency on any specific trait varied depending on the population (donor). Under normal irrigated conditions in Hainan, the 64 ILs selected for HY in Beijing had an average yield of 24.9 g per plant, or 13.2% higher than HHZ (Table 2). Of these, 8 ILs had significantly higher GY than HHZ, resulting primarily from increased SNP/FNP (Table 3). The remaining ILs had the same GY as HHZ.