Some examples are discussed beneath. The strongest enrichment of function is seen for the ribosomal proteins around the left hand edge on the map. However, there’s a second group of ribosomal protein genes in the centre with the map that may be characterised by high expression in ovaries and is hence likely to become involved in egg production. Genes involved in DNA, RNA and protein synthesis are usually discovered above the diagonal from reduced left to upper right. Temporally, spatially or functionally associated metabolic functions are usually co situated around the map. One example is, near the centre on the map, clusters enriched in protein synthesis, protein folding and protein degradation are discovered with each other. Additional file 1, Figure S1 shows a wider collection of DNARNAprotein metabolic functions and their relationships.
1 further gene function analysis was performed. The null hypothesis asserts that the genes annotated having a distinct selleckchem GO term are randomly distributed across the map, and that is tested empirically. Table three lists the 59 GO terms for which this null hypothesis is rejected at P 0. 01, that’s, functions which are non randomly distributed on the map. Multi vs. single experiment maps A few of the experiments integrated within this analysis, for example the life cycle series, blood meal time series and embryonic developmental series, possess a large adequate number of experimental circumstances to permit efficient clustering of genes on their very own. Using an identical self organizing map strategy on information from these individual experiments, we discovered considerable enrichment of gene function in the resulting clusters, as shown in Figure 3.
Even so the amount of enriched GO terms was lower than that obtained using the all experiment map. The selleck chemical differ ence is specifically clear in the P 110 6 threshold. The mapping was also performed with two older releases on the expression information, which contain fewer experiments. Normally there’s a rise in the variety of enriched GO terms as new experiments are added, although there is a little drop within the number of biological approach terms from release 1. 0. 3 to 1. 0. five. From this restricted information we tentatively pre dict that the incorporation of future experimental information will produce increasingly informative maps. Additional dis cussion around the inclusionexclusion of experiments is often located beneath. Case study odorant binding proteins Odorant binding proteins, which transport odorant molecules through the extracellular fluid of che mosensilla to transmembrane odorant receptor proteins on olfactory receptor neurons, are located in 3 principal regions on the expression map. One particular region is characterised by minimal differential regulation and presumably represents constitutively expressed genes.