In contrast to a lack of tumor formation in controls, 62% of Ptc1

In contrast to a lack of tumor formation in controls, 62% of Ptc1/ mice and 67% of Gli31/ mice designed medulloblastomas. Our information indicate that mis expression of Shh alone while in the postnatal brain is not really sufficient to induce tumors effi ciently. Nonetheless, by also getting rid of one copy of Ptc or Gli3, medulloblastomas kind at a significantly higher charge. This examine represents the very first proof that Gli3 could ordinarily perform a position inside the repression of medulloblastoma formation. PE 24. NOVEL CELL LINES FROM PEDIATRIC BRAIN TUMORS Present MULTI DRUG RESISTANCE Jingying Xu, Ignacio Gonzalez Gomez, Elizabeth Y. Melendez, Rex A. Moats, Anat Erdreich Epstein, and C. Patrick Reynolds, Developmental Therapeutics Plan, USC UCLA Institute for Pediatric Clinical Analysis, Division of Hematology Oncology, Childrens Hospital Los Angeles and Departments of Pediatrics and Pathology, Keck College of Medicine, University of Southern California, Los Angeles, CA, USA The paucity of cell culture models for childhood brain tumors has prompted us to establish this kind of cell lines for use in biological and preclini cal developmental therapeutic studies.
Brain tumors from pediatric patients had been obtained at surgical procedure or publish mortem and minced and cultured selleckchem in lscoves Dulbeccos Modified Eagles Medium. Here we describe three new cell lines, CHLA 200, CHLA 259 and CHLA 266. All 3 cell lines had unique DNA fingerprints based on the ABI Profiler. All cell lines had been adherent on tissue culture plastic but differed in morphology. The doubling occasions have been 76 hours for CHLA 259, 45 hours for CHLA 266, and fifty five hrs for CHLA 200 com pared with 29 hours to the commonly used medulloblastoma cell line DAOY. In contrast to fibroblasts, these four cell lines expressed telomerase reverse transcriptase and telomerase RNA template.
CHLA 266, CHLA 259, and CHLA 200 strongly expressed the early neuronal marker PGP9. five but did not express tyrosine hydroxylase. DAOY, CHLA 259, and CHLA 266 expressed MYCN mRNA, whereas CHLA 266 and CHLA 200 expressed cmyc. The cell Tivozanib lines had been injected to the brains of nude mice, but only DAOY and CHLA 266 have verified tumorigenic to date. All 3
s showed striking multidrug resistance when in contrast with DAOY cells using a fluorescein diacetate and digital picture microscopy cytotoxicity assay. The table exhibits concentrations of medicines which can be lethal for 90% of cells. CHLA 271 and CHLA 280 are new cell lines. These new brain tumor cell lines will produce valuable versions for preclinical testing of novel medicines for probable activity against multidrug resistant pediatric brain tumors. This examine was supported through the Devin Hock Memorial Fund of your Michael Hoefflin Foundation as well as the Pediatric Brain Tumor Foundation. PE 25. LINEAGE Limited PROGENITORS CAN SERVE AS CELLS OF ORIGIN FOR MEDULLOBLASTOMA Zeng Jie Yang,one Tracy Ann Go through,one Tammy Ellis,2 Robert Machold,3 Gord Fishell,3 David H.

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